Autoclaves

All new users of the autoclaves must be trained by Michael Arlt. Once training is complete it is added to the autoclave training record.

Autoclaves can be found in two areas in the Earth Sciences Centre:

Basement, Room B120
First Floor, Room ES1023A (Teaching)

For assistance, see Michael Arlt in room ES1023C

How do we autoclave garbage?

There are three autoclaves in the basement, each numbered individually. Autoclave#1 has been designated for autoclaving garbage. All other autoclaves in the department are NOT to be used for sterilizing garbage.

For all Bio Safety Level I labs in the building, when autoclaving garbage, use only clear autoclave bags with NO biohazard symbol on the bad to sterilize all biological material (i.e. petri plates, cultures, etc.).

  • Do not OVERFILL the bag. Leave enough room to close the bag in a safe manner.
  • Do not SEAL the bag tightly. Leave an opening to allow steam formed inside the bag to escape.
  • Place the autoclave bag in a tub, and only then start autoclaving. HOT MEDIA INSIDE THE AUTOCLAVE WILL PLUG-UP THE DRAIN.
  • The material coming out of the autoclave is VERY HOT. Use oven gloves to protect your hands.
  • Once the garbage has been autoclaved, let the material cool for several hours and then place the bag in a regular large garbage can.

For all Bio Safety Level II labs, garbage is autoclaved in bags printed with a biohazard symbol. After autoclaving, these bags are then brought back to the individual research labs, and placed in containers provided by the Environmental Protection Services at UofT. The containers are then picked up when the Environmental Protection Services is contacted by the lab.

How to autoclave and dispose of contaminated sharp materials?

Autoclave all sharp items such as contaminated broken glassware, pipettes, or razor blades in SEPARATE AUTOCLAVE BAGS. After autoclaving, dispose of the bags containing the sharps into one of the Lab Glass and Plastic Totes supplied by Recycling in your lab and the containers with blades and needles contact Environmental Protection Services (416-946-3473) for proper disposal.